Figure 2.

(A) Sequence alignment of the second intracellular loop of mGluRs; the region involved in selective coupling of mGluR1α with G_q/11 is boxed in gray. An asterisk highlights Thr⁶⁹⁵, which we propose to be substrate for PKC-mediated phosphorylation. Basic amino acid residues which can constitute the consensus for PKC are underlined. (B) Effect of PMA and bisindolylmaleimide I (Bis) on the Thr⁶⁹⁵Ala mutant receptor activity. Cell transfection and treatment were conducted as described for the wild-type receptor (Fig. 1A). Results represent means ± SEM of duplicate or triplicate determinations obtained from at least two independent experiments (*, P ≤ 0.05; two-tailed t test).