Figure 3.

Rapid desensitization of mGluR1α depends on the phosphorylation state of Thr⁶⁹⁵. (A) Representative tracings of currents generated by wild-type and mutant receptors expressed in oocytes upon repeated application of Glu (1 mM); arrows indicate beginning of Glu application which lasted for 1 min. Time scale: 1 min. (B–F) PKC dependence of wild-type and mutant receptor responses. Injected oocytes were incubated with either staurosporin (100 nM) or bisindolylmaleimide I (2 μM) or PMA (100 nM) before recordings. Results are means ± SEM of values obtained from two independent experiments (*, P ≤ 0.05; **, P ≤ 0.01; two-tailed t test).