Table 1.
Medium | Apoptotic nuclei, % of total cells |
---|---|
K+ (25 mM) | 5.0 ± 0.3 |
K+ (5 mM) | 29.3 ± 2.0 |
K+ (5 mM) + GROβ | 16.0 ± 0.9 |
Six-day cultured cells were kept for a further 24 h in 5 mM K+-containing medium in the presence or in the absence of 60 nM GROβ, and treated with the fluorescent dye Hoechst 33258. Data indicate the number of apoptotic nuclei as percentage of total cells and represent the mean ± SEM of four independent experiments. Student's t test paired analysis of K+ 5 mM vs. K+ 5 mM + GROβ-treated cells: P < 0.0001.