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. 2000 May 16;97(11):6207–6211. doi: 10.1073/pnas.110137497

Figure 6.

Figure 6

Effects of treatment of DES on OTR mRNA and immunoreactive OT in the aorta and vena cava of immature female rats. (A) OTR mRNA was determined by semiquantitative RT-PCR. Total RNA (1 μg) was reverse transcribed and cDNA was exponentially amplified by PCR in the presence of gene-specific primers. PhosphoImager scan (upper panel) is presented after separation of radioactive PCR product by electrophoresis in 1.5% agarose. The bar graphs present the OTR mRNA level verified to GAPDH mRNA PCR product used as internal standard and calculated as a ratio of signal given by radioactive bands OTR mRNA PCR product (n = 4, *P < 0.05). (B) Comparison of OT content in the aorta and vena cava in control and DES-treated rats by RIA.