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. Author manuscript; available in PMC: 2008 Jan 4.
Published in final edited form as: Neuron. 2007 Jan 4;53(1):79–89. doi: 10.1016/j.neuron.2006.11.024

Figure 5. Impaired activation of MAPK and PKA following contextual fear conditioning in DKO mice.

Figure 5

(A) Western blot analysis of hippocampal pERK in WT and DKO mice after training (5–10 min). Blot shows pooled samples; the graph shows mean data from individually run samples. U, unpaired; P, paired. (B) Immunohistochemical analysis of pERK in DKO mice after training (30 min). Representative images of the CA1 region. Scale bar, 50 μm. (C) Western blot analysis of total ERK expression in WT and DKO hippocampi. (D) Average intensity levels of pPKA-s in pERK- and pERK+ CA1 somata from WT and DKO hippocampi after training (30 min). (E) Ratio of the pERK labeling intensity in the nucleus relative to the cytoplasm in WT and DKO mice.