Table 3.
Bacterial strains used in this study.
| Strain | Relevant genotype | Reference or construction (parents; relevant selection) |
|---|---|---|
| Wild type strain | ||
| RP437 | F’ thi-1 (Am) leuB6 hisG4 metF159(Am) eda rpsL 136 lacY1 mtl-1 tonA31 tsx-78 xyl-5 | (Parkinson and Houts, 1982) |
| Aerotaxis mutants | ||
| RP5882 | Δtsr-7021 | (Callahan et al., 1987) |
| UU117 | Δaer-1 | (Bibikov et al., 1997) |
| BT3409 | Δtsr-5550:erm | RP437[pRK1; Emr]a |
| BT3312 | Δaer-1 Δtsr-7021 | (Repik et al., 2000) |
| Electron transport system mutants | ||
| AJW851 | CP875 nuoF::miniTn10Cm | (Falk-Krzesinski and Wolfe, 1998) |
| GO103 | GR70N Zbg-2200::kan Δ(cydAB’)455 | (Oden et al., 1990) |
| GO104 | GR70N Δ(cyoABCDE)456::kan | (Calhoun and Gennis, 1993) |
| BT3389 | Δaer-1 ndh:erm | UU1117[pKJW1; Emr]b |
| BT3390 | Δtsr-7021 ndh:erm | RP5882[pKJW1; Emr]b |
| BT3414 | Δaer-1 nuoF::miniTn10Cm | AJW851 5 UU1117; Cmr |
| BT3415 | Δtsr-7021 nuoF::miniTn10Cm | AJW851 5 RP5882; Cmr |
| BT3401 | BT3414 Δ(cyoABCDE)456::kan | GO104 5BT3414; Cmr/Knr |
| BT3402 | BT3415 Δ(cyoABCDE)456::kan | GO104 5BT3415; Cmr/Knr |
| BT3403 | BT3414 Zbg-2200::kan Δ(cydAB’)455 | GO103 5BT3414; Cmr/Knr |
| BT3404 | BT3415 Zbg-2200::kan Δ(cydAB’)455 | GO103 5BT3415; Cmr/Knr |
| BT3405 | BT3389 Δ(cyoABCDE)456::kan | GO104 5BT3389; Emr/Knr |
| BT3406 | BT3390 Δ(cyoABCDE)456::kan | GO104 5BT3390; Emr/Knr |
| BT3407 | BT3389 Zbg-2200::kan Δ(cydAB’)455 | GO103 5BT3339; Emr/Knr |
| BT3408 | BT3390 Zbg-2200::kan Δ(cydAB’)455 | GO103 5BT3390; Emr/Knr |
a
The tsr gene was replaced by allelic exchange with the temperature sensitive plasmid pRK1, constructed by R. Korson.
b
The ndh gene was replaced by allelic exchange with the temperature sensitive plasmid pKJW1, constructed by K. Watts.