Figure 4. Formation of MIG-5::GFP puncta prior to and after the B cell division was dependent uponlin-17 but not lin-44.

MIG-5::GFP accumulated at the anterior cortex of the B cell in wild-type males (A) and at the posterior cortex of B.a and B.p cells after division in wild type males (B) in a punctate pattern (small arrows). Posterior B.p puncta are slightly below the focal plane. MIG-5::GFP puncta were not observed in lin-17 males (C). In lin-44 males, prior to cell division, both anterior and posterior cortex of B cell displayed weak puncta (D) and after division, the anterior cortex of B.a and B.p cells displayed puncta (E). However, the MIG-5 puncta in lin-44 males were often mislocalized. Bar equals 10 μm in all panels.