Table 3.

Primers used in this work

Primer name	Primer sequencea
Genetic Replacement Study
SOEA	AAC TGC AGT TAT ATA TTT GCG GC
SOEB	GAT ATG AAC CTC CTT ATT
SOE1	AAT AAG GAG GTT CAT ATC ATG AAA AGT TCT TTT TTA
SOE2	TTC ATT TTT TAC TCC TAG CAA GCG CGC GTA CAA GC
SOE3	TAG GAG TAA AAA ATG AA 3
SOED	ACG AAT TCT CTT ACA GAG TT
SOE6	GAAGATGTATTTGGTGCGTGTAGTACAAACACTTTT
SOE7	CGCACCAAATACATCTTC
SOE4	ATTAATTAAAAATTTGTTATTAACTTATTTACACCAAGACAT
SOE5	GTTAATAACAAATTTTTAATTAAT
SOE8	GAGTCTCATGGAGGAGGAACACCTTTAGCCTTATTG
SOE9	TGTTCCTCCATGAGACTC
Check primers genetic replacement study
CheckC55α1_for	AACACTTTTTCTTTGAGTG
CheckC55α_rev	GACATACATTCGTGAGTA
1CheckC55α_rev	ACAAGCTCCAAAAATATCA
CheckC55β2_for	ACCGGGGTGATAGGTTAT
1CheckC55β_for	TACTGATGATGAAGTATATGC
CheckC55β_rev	ATATAACCTATCACCCCG
CheckltnR_for	GAGATAGTACTCTGTAC
CheckltnM1_rev	GATTTATCTTCTCAATG
Residue Specific Mutagenesis
N15K_for	CTCAGTGATTACTGGGGAAATTTAGGGGCTTGGTGTACACTCAC
N15K_rev	GTGAGTGTACACCAAGCCCCTAAATTTCCCCAGTAATCACTGAG
A17N_for	GATTACTGGGGAAATAACGGGAACTGGTGTACACTCACTCATG
A17N_rev	CATGAGTGAGTGTACACCAGTTCCCGTTATTTCCCCAGTAATC
L21A_for	AATAACGGGGGGTGGTGTACAGCAACTCATGAATGTATGGCTTG
L21A_rev	CAAGCCATACATTCATGAGTTGCTGTACACCACCCCCCGTTATT
A27S_for	CACTCACTCATGAATGTATGTCGTGGTGTAAATAAGTTAATAAC
A27S_rev	GTTATTAACAATTTTACACCACGACTAACATTCATGAGTGAGTG
Check primers residue specific mutagenesis
CheckN15K_for	GATTACTGGGGAAATTTA
CheckA17N_for	TGGGGAAATAACGGGAAC
CheckL21A_for	GGGGGGTGGTGTACAGCA
CheckA27S_for	ACTCATGAATGTATGTCG
CheckSDM_rev	TGTCGAATTCATTTCTGGAAAAAC

a. Primer sequences are given in the 5' to 3' direction