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. 2007 May 1;5(5):e110. doi: 10.1371/journal.pbio.0050110

Figure 3. Mild Uncoupling by DNP Prolongs Replicative Lifespan of Human Fibroblasts.

Figure 3

MRC5 fibroblasts were treated with 250 μM DNP from PD 35 till senescence.

(A) MitoSOX fluorescence. Data are mean + Δm, n = 2. Fluorescence intensity at t = 0 was set to 100%. Solid line: linear regression, dotted lines: 95% confidence intervals.

(B) Replicative lifespan. Data are from three (DNP) respective four (control) independent experiments. Hyperbolic best fits and 95% confidence intervals are shown.

(C) γ-H2A.X staining. The bar denotes 20 μm. Insert: higher magnification showing focal staining.

(D) Frequency of γ-H2A.X-positive fibroblasts. Data are mean ± s.d., n = 3. Differences are significant (p < 0.05) between day 8 and 46.