Figure 4.
(A) Western blots of root protein extracts from transgenic tobacco plants. Rabbit polyclonal anti-P450 2E1 was used as the primary antibody. Lanes 1 to 8 are extracts from plants 3–1, 3–3, 3–5, human P450 2E1 (2 ng), 3–6, 3–7, 3–8, respectively. A 15-μl aliquot of extract (equivalent to 7.5 mg of fresh weight) was loaded in each lane except for the P450 2E1 standards. The arrowheads indicate the position of P450 2E1. (B) Correlation between trichloroethanol and P450 2E1 concentrations in transgenic tobacco roots. Amounts of P450 2E1 protein in root extracts were calculated based on a quantative Western blot standard curve (R2 = 0.988), and the nanogram values were converted to concentrations based on the fresh weight of the tissue from which the extracts were prepared.