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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Dev Biol. 2006 Oct 25;303(2):772–783. doi: 10.1016/j.ydbio.2006.10.030

Figure 7.

Figure 7

Trunk circulation and intersegmental vessel sprouting defects in zebrafish injected with morpholinos targeting vascular ETS factors. Morpholinos targeting fli1, fli1b, ets1, or etsrp were injected into Tg(fli1:EGFP)y1 zebrafish either alone, in combinations of three morpholinos, or all together, as noted by “x” marks at the top and bottom of the figure. The number of animals for which phenotypes were assessed for each injection is shown at the extreme bottom of the Fig. (N=). The upper graph shows the % of animals with active blood circulation in the trunk at 24 hpf (purple bars) and 48 hpf (red bars). The middle graph shows the % of animals at 24 hpf with 1–15 (orange bars) or >15 (blue bars) intersegmental vessel sprouts in the trunk proper, as visualized by microscopic examination of fli1:EGFP transgene expression in developing blood vessels. The lower graph shows the % of animals at 48 hpf with 1–15 (orange bars) or >15 (blue bars) intersegmental vessel sprouts in the trunk proper. The graphs also include tabulation of circulation and intersegmental vessel sprout phenotypes measured in control morpholino injected animals, all of which had trunk circulation and a full complement of trunk intersegmental vessel sprouts.