Fig. 5.

Expression of IKKα transgene enhances terminal differentiation and reduces mitogenic activity. (A) Filaggrin levels in WT and Lori·IKKα-Tg (Tg) skin treated with DMBA/TPA for 19 weeks. Relative ratio, densities of filaggin signals normalized by controls (β-actin); β-actin, loading control. (B) Levels of Raf and ERK activity in WT and Tg skin treated with DMBA/TPA for 19 weeks. β-actin, loading control. (C) Ki67-positive cell numbers in epidermis from WT and Tg mice treated with DMBA/TPA for 28 weeks. Seven slides (15 views in each slide) from WT mice and seven slides from Tg mice were counted. (D) Skin of WT and Tg mice stained with an anti-Ki67 antibody. Dark brown color, Ki67-positive cells. (Original magnification: ×200.)