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. 2006 Nov 6;103(46):17266–17271. doi: 10.1073/pnas.0608156103

Fig. 2.

Fig. 2.

The effects of SC1 on known self-renewal pathways. (A) Western blot analysis of STAT3 phosphorylation at Tyr-705. OG2-mES cells were cultured in the ESC-N2B27 media overnight and then stimulated with DMSO (negative control), 1 μM SC1, 1 μM SC1− (negative control of SC1), 103 units/ml LIF (positive control), or 1 μM SC1 plus 103 units/ml LIF for 20 min. (B) Quantitative RT-PCR analysis of Id1 gene expression. OG2-mES cells were cultured in the ESC-N2B27 media overnight and then stimulated with DMSO (negative control), 1 μM SC1, or 50 ng/ml BMP4 (positive control) for 45 min. (C) Wnt signaling by Super (8×) TOPflash reporter assay. Stable Super (8×) TOPflash reporter 293T cells were treated with DMSO (negative control), 3 μM SC1, or 2 μM BIO (positive control) for 48 h and assayed for luciferase activity. (D) Transient activation of Nanog expression. OG2-mES cells were treated with 1 μM SC1 in the ESC-N2B27 media, and mRNAs were isolated at 0, 6, and 18 h, separately. In these experiments, OG2-mES cells were seeded at the same density (1.6 × 104 cells/cm2). PD, PD098059; SC1−, negative control of SC1.