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. 2006 Nov 3;103(46):17337–17342. doi: 10.1073/pnas.0607015103

Fig. 1.

Fig. 1.

dsRNAs targeting the E-cadherin gene promoter induce E-cadherin mRNA and protein expression. (A) A schematic representation of the E-cadherin promoter with its CpG island, Alu repeat element, transcription start site, and dsRNA targets. (B) PC-3 cells were transfected with 50 nM dsRNA for 72 h. mRNA expression of E-cadherin and GAPDH were analyzed by RT-PCR. Samples for each treatment are shown in duplicate. (C) E-cadherin, β-actin, and GAPDH protein levels were detected by Western blot analysis in PC-3 cells treated as in B. (D) PC-3 cells were treated with dsEcad-215 at the indicated concentrations for 72 h. E-cadherin and GAPDH expression was detected by Western blotting. (E) PC-3 cells were transfected with 50 nM dsEcad-215 for the indicated lengths of time. E-cadherin and β-actin expression was detected by Western blotting. (F) PC-3 cells were transfected with 50 nM dsCon-1 or dsEcad-215 for the indicated periods of time. Mock samples were transfected in the absence of dsRNA. Western blot analysis shows E-cadherin protein levels in PC-3 cells on days 10 or 13 after single transfections.