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. 2006 Nov 3;103(46):17337–17342. doi: 10.1073/pnas.0607015103

Fig. 4.

Fig. 4.

Sequence specificity for RNAa. (A) Sequence for E-cadherin dsRNA dsEcad-215. The antisense (guide) strand is shown in blue. Mutation of the first and last 5 bp of the dsEcad-215 duplex resulted in dsEcad-215-G3′ and dsEcad-215-G5′, respectively. The mutated bases are in red. (B) PC-3 cells were transfected with 50 nM of the indicated dsRNA molecules for 72 h. Expression of E-cadherin and β-actin was assessed by Western blot analysis. (C) E-cadherin levels from corresponding Western blots were normalized to β-actin and are presented as the mean ± SEM of two independent experiments. (D) The sequence of dsP21–322 and its corresponding mutated dsRNAs. (E) PC-3 and HeLa cells were transfected with 50 nM of the indicated dsRNA molecules for 72 h. Expression levels of p21 and GAPDH were assessed by Western blot analysis.