Fig. 2.
HSVTLR2* variants are recognized through TLR2, TLR1, and TLR6 independently of CD14. (A) HEK293T cells were transfected with TLR2 and/or CD14 plasmid. The cells were stimulated with 5 MOIe of UV-inactivated HSV-1 KOS-K, 10 μg/ml of zymosan, or 5 μg/ml of CpG 1826 for 7 h. Luciferase activity was measured. (B) 293T/luc/TLR2/CD14 cells were transfected with 500 ng of DN TLR1 or DN TLR6 plasmid and Renilla luciferase plasmid. Twenty-four hours later, the cells were stimulated with 5 MOIe of UV-inactivated HSV-1 KOS-K or zymosan for 7 h. Relative luciferase unit was calculated as a ratio of NFκB-dependent firefly luciferase activity to NFκB-independent Renilla luciferase activity. N/D, not detected. ∗, P < 0.05. The data are representative of three similar experiments.