Fig. 5.

Direct virus recognition, but not bystander stimulation, induces cytokine secretion from cDCs. IL-6^−/− BM DCs (stimulator) were incubated with 2 MOI of live or UV-inactivated HSV-1_TLR2* or HSV-1_TLR2° for 24 h, and after thorough washing, they either were incubated for additional 24 h for assessment of IL-12 secretion (A) or exposed UV light to prevent de novo viral replication. (B) The stimulator DCs were cocultured with the indicated responder DCs. (C) In parallel, the responder DCs alone were incubated directly with live or UV-inactivated HSV-1_TLR2* or HSV-1_TLR2° for 24 h, and IL-6 levels were measured. The data are representative of three similar experiments.