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. 2006 Nov 6;103(46):17349–17354. doi: 10.1073/pnas.0603079103

Fig. 2.

Fig. 2.

The CycT1-binding domain, but not BR, in Hexim1 inhibits CIITA-mediated transcription. (a) Deletion mutant Hexim1 proteins used in CAT assays. Numbers to the right mark boundaries of wild-type and mutant Hexim1 proteins. The white box represents the BR, the gray box marks the TBD, and the white oval depicts the nuclear localization signal (NLS). (b) Hexim1 with the intact TBD represses the activity of CIITA. Experiments were performed as in Fig. 1a, with the same amounts of pDRASCAT (lane 1), f:CIITA.IF1 (lane 2), and deletion mutant Hexim1 proteins. f:Hex1(1–200) (lanes 3–5), f:Hex1(150–359) (lanes 6–8), and f:NLS.Hex1(181–359) (lanes 9–11). Images below the bar graph represent expression of f:CIITA.IF1, truncated Hexim1 proteins, and GAPDH as determined by Western blotting.