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Hexim1 competes with the CIITA for the binding to CycT1 in vitro. The GST.CycT1 chimera was expressed in E. coli and purified from the lysate. f:CIITA.IF1 together with f:Hex1 were synthesized as described in Materials and Methods. f:CIITA.IF1 and f:Hex1 were incubated alone with GST.CycT1 chimera (lanes 1 and 2) or in combination, with a 7-fold excess of f:Hex1 (lane 3). Lanes 4 and 5 represent input for f:CIITA.IF1 and f:Hex1 used in competition assays.
