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. 2006 Nov 6;103(46):17432–17437. doi: 10.1073/pnas.0607939103

Fig. 3.

Fig. 3.

Constitutive activation of NF-κB inhibits osteogenic differentiation of arthritic rFLS. RV-transduced cells (as in Fig. 2B) were incubated in a control medium (DMEM) or in an osteogenesis-inducing medium (OS). (A) The activity of ALP in the control (Babe) and in RelA-transduced cells after 14 days of incubation. (B) ALP activity in the control and IKK(EE)-transduced cells after 14 days of incubation. (C) Mineral deposition in the control (Babe) and in IKK(EE)-transduced cells after 21 days of incubation, as assessed by silver staining. (Original magnification in A and C: ×100.) (D) Calcium deposition in cells shown in C was quantitatively assessed and normalized on protein content. (E) The expression of an osteogenic differentiation marker (osteopontin) in cells shown in B as assessed by RT-PCR.