Fig. 4.

ccd-Angptl-4 bound with high affinity to dimeric, but less to monomeric, LPL as determined by surface plasmon resonance. Biotinylated dimeric and monomeric LPL were bound to streptavidin-coated sensor chips to a level of 5,100 response units (RU) and 5,300 RU, respectively. ccd-Angptl-4 (13.8 μg/ml) was injected over these two flow cells and over a flow cell that contained only streptavidin (1st injection). At the indicated time (arrow) the flow was changed to buffer without ccd-Angptl-4 to study dissociation of the ligand. Then a second injection of the same ccd-Angptl-4 solution was made (2nd injection). The buffer contained 20 mM Hepes and 0.15 M NaCl and the temperature was 25°C.