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. 2007 Mar 1;189(3):241–249. doi: 10.1111/j.1365-201X.2006.01662.x

Figure 4.

Figure 4

Relative fluorescence against time (s). Cells from control (a) and 24-h ligated glands (b) were stimulated with increasing doses of methacholine (10−8–10−6m). Cells were then incubated with ionomycin (iono) and then manganese chloride (Mn2+) to elicit the maximum and minimum fluorescence signal. During stimulation by methacholine a biphasic response was seen comprising a peak (1) and plateau (2) phase.