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PHO5 promoter remodeling is delayed when RNAPII elongation is impaired. (A) Relative histone H3 density (H3C ChIP) in the indicated regions of the PHO5 promoter (UASp2 and TATA) in WT and dst1 cells during phosphate starvation, with or without 6AU treatment to inhibit RNAPII transcript elongation. (B) As in A, but RNAPII recruitment to the TATA box (4H8 ChIP). Density at time = 0 was set to 1, and other values are expressed relative to that.
