Fig. 5.

Abolishing the intergenic transcript leads to slower PHO5 promoter remodeling and RNAPII recruitment to the PHO5 TATA box. (A Upper) Schematic of the PHO5 locus in WT and PHO5-3′Δ. URA3 replaces PHO5 +751–1,404 (to end of gene). (A Lower) Northern blot (using probe 3 from Fig. 1C) showing absence of intergenic transcription in the rrp6 version of the strain is shown. Note that only RRP6 cells were used in B and C and other experiments addressing the functional consequences of intergenic transcription. (B) Relative histone H3 occupancy (H3C ChIP) in the indicated regions of the PHO5 promoter in WT and PHO5-3′Δ cells during the course of phosphate starvation. (C) As in B, but RNAPII recruitment to the TATA box (4H8 ChIP). Density at time = 0 was set to 1, and other values are expressed relative to that. Histone density in the two strains at time = 0 was similar.