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. 2007 Feb 5;9(1):R12. doi: 10.1186/ar2119

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Copyright © 2007 Schilders et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cleavage of the PM/Scl-75 protein during anti-Fas-induced apoptosis. (a) Jurkat cells were treated with the anti-Fas monoclonal antibody 7C11 for the indicated durations. Protein extracts were analyzed by 10% SDS-PAGE and Western blotting with a polyclonal anti-PM/Scl-75 rabbit serum. (b) Jurkat/Neo and Jurkat/Bcl-2 cells were treated with the anti-Fas monoclonal antibody 7C11 for the indicated durations. Protein extracts were analyzed by 10% SDS-PAGE and Western blotting with the anti-PM/Scl-75 rabbit serum. In each panel the positions of molecular mass markers are indicated in kDa at the right and the positions of the relevant polypeptides are shown at the left. Filled arrowheads indicate full-length proteins, open arrowheads indicate cleavage products and the asterisk indicates a cross-reactivity of the anti-PM/Scl-75 serum with an unknown protein.