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Signalling pathways of inhibition by galectin-3 (gal-3) of vitamin D3-stimulated osteocalcin production. Osteoblasts were treated with 5 μg/ml of recombinant human gal-3 in the presence of vitamin D3 and osteocalcin was determined. Inhibitor concentrations were: KT5720, 2 μM; KT5823, 2 μM; Genistein (Genist.), 1 μM; Taxifollin, 1 μM; wortmannin (Wortma.), 250 nM; PD98059, 10 μM; and SB202190, 2 μM. *P versus the autologous control; n = 5.
