Table 1 Source and dilution of antibodies.
| Antigen | Antibody clone | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|
| SMA | 1A4 | 1/100 | None | Dako (Glostrup, Denmark) |
| Desmin | D33 | 1/100 | Microwave (EDTA, Tris, citrate pH 8.0) | Dako (Glostrup, Denmark) |
| S100 protein | Polyclonal | 1/500 | None | Dako (Carpinteria, California, USA) |
| HMB45 | HMB45 | 1/100 | None | Dako (USA) |
| Vimentin | V9 | 1/100 | Microwave (EDTA, Tris, citrate pH 8.0) | Dako (Denmark) |
| CD34 | QBEnd 10 | 1/50 | Microwave (EDTA, Tris, citrate pH 8.0) | Dako (Denmark) |
| CK | AE1/AE3 | 1/100 | Protease digest (proteinase K) | Dako (USA) |
CK, cytokeratin; SMA, smooth muscle actin.