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. 1983 Feb;23(2):232–235. doi: 10.1128/aac.23.2.232

pH-dependent oxacillin tolerance of Staphylococcus aureus.

J S Venglarcik 3rd, L L Blair, L M Dunkle
PMCID: PMC186027  PMID: 6551162

Abstract

A proportion of clinical isolates of Staphylococcus aureus exhibit resistance to bactericidal activity of certain antibiotics, despite normal susceptibility to inhibition. This phenomenon is termed "tolerance." The methodology used to determine tolerance varies greatly. To clarify the relationship between laboratory methodology and tolerance, we determined the minimal bactericidal concentrations and minimal inhibitory concentrations for 20 clinical isolates by two methods. Inocula were prepared by either 3-h growth of the organism in Mueller-Hinton broth or overnight (22 to 24 h) cultures in Trypticase soy broth (BBL Microbiology Systems, Cockeysville, Md.). All inocula were plated for colony counts and tested for pH. An American Type Culture Collection (Rockville, Md.) reference strain was included in all tests for standardization. Tolerance was defined by the strictest criterion, i.e., a minimal bactericidal concentration/minimal inhibitory concentration ratio of greater than or equal to 100. With the first method, none of the 20 isolates displayed tolerance (mean inoculum pH, 7.15). When inocula were grown in Trypticase soy broth with overnight incubation, 35% (7 of 20) showed tolerance (mean inoculum pH, 6.22). There was a significant association between the decreased bactericidal capacity at high oxacillin concentrations and overnight incubation in Trypticase soy broth (P less than 0.01). We suggest that tolerance in staphylococci is in some way related to the pH value of the inoculating culture. Such pH-induced tolerance may have a clinical corollary in sequestered infections where the pH is acidic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradely J. J., Mayhall C. G., Dalton H. P. Incidence and characteristics of antibiotic-tolerant strains of Staphylococcus aureus. Antimicrob Agents Chemother. 1978 Jun;13(6):1052–1057. doi: 10.1128/aac.13.6.1052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley H. E., Weldy P. L., Hodes D. S. Tolerance in Staphylococcus aureus. Lancet. 1979 Jan 20;1(8108):150–150. doi: 10.1016/s0140-6736(79)90536-1. [DOI] [PubMed] [Google Scholar]
  3. Goldman P. L., Petersdorf R. G. Significance of methicillin tolerance in experimental staphylococcal endocarditis. Antimicrob Agents Chemother. 1979 Jun;15(6):802–806. doi: 10.1128/aac.15.6.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goodell E. W., Lopez R., Tomasz A. Suppression of lytic effect of beta lactams on Escherichia coli and other bacteria. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3293–3297. doi: 10.1073/pnas.73.9.3293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hilty M. D., Venglarcik J. S., Best G. K. Oxacillin-tolerant staphylococcal bacteremia in children. J Pediatr. 1980 Jun;96(6):1035–1037. doi: 10.1016/s0022-3476(80)80633-0. [DOI] [PubMed] [Google Scholar]
  6. Houtsmuller U. M., van Deenen L. L. On the amino acid esters of phosphatidyl glycerol from bacteria. Biochim Biophys Acta. 1965 Dec 2;106(3):564–576. doi: 10.1016/0005-2760(65)90072-x. [DOI] [PubMed] [Google Scholar]
  7. Ishida K., Guze P. A., Kalmanson G. M., Albrandt K., Guze L. B. Variables in demonstrating methicillin tolerance in Staphylococcus aureus strains. Antimicrob Agents Chemother. 1982 Apr;21(4):688–690. doi: 10.1128/aac.21.4.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kim K. S., Anthony B. F. Importance of bacterial growth phase in determining minimal bactericidal concentrations of penicillin and methicillin. Antimicrob Agents Chemother. 1981 Jun;19(6):1075–1077. doi: 10.1128/aac.19.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lopez R., Ronda-Lain C., Tapia A., Waks S. B., Tomasz A. Suppression of the lytic and bactericidal effects of cell wallinhibitory antibiotics. Antimicrob Agents Chemother. 1976 Oct;10(4):697–706. doi: 10.1128/aac.10.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mayhall C. G., Apollo E. Effect of storage and changes in bacterial growth phase and antibiotic concentrations on antimicrobial tolerance in Staphylococcus aureus. Antimicrob Agents Chemother. 1980 Nov;18(5):784–788. doi: 10.1128/aac.18.5.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mayhall C. G., Medoff G., Marr J. J. Variation in the susceptibility of strains of Staphylococcus aureus to oxacillin, cephalothin, and gentamicin. Antimicrob Agents Chemother. 1976 Oct;10(4):707–712. doi: 10.1128/aac.10.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pearson R. D., Steigbigel R. T., Davis H. T., Chapman S. W. Method of reliable determination of minimal lethal antibiotic concentrations. Antimicrob Agents Chemother. 1980 Nov;18(5):699–708. doi: 10.1128/aac.18.5.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peterson L. R., Gerding D. N., Hall W. H., Schierl E. A. Medium-dependent variation in bactericidal activity of antibiotics against susceptible Staphylococcus aureus. Antimicrob Agents Chemother. 1978 Apr;13(4):665–668. doi: 10.1128/aac.13.4.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rajashekaraiah K. R., Rice T., Rao V. S., Marsh D., Ramakrishna B., Kallick C. A. Clinical significance of tolerant strains of Staphylococcus aureus in patients with endocarditis. Ann Intern Med. 1980 Dec;93(6):796–801. doi: 10.7326/0003-4819-93-6-796. [DOI] [PubMed] [Google Scholar]
  15. Raynor R. H., Scott D. F., Best G. K. Oxacillin-induced lysis of Staphylococcus aureus. Antimicrob Agents Chemother. 1979 Aug;16(2):134–140. doi: 10.1128/aac.16.2.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogers H. J., Forsberg C. W. Role of autolysins in the killing of bacteria by some bactericidal antibiotics. J Bacteriol. 1971 Dec;108(3):1235–1243. doi: 10.1128/jb.108.3.1235-1243.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sabath L. D., Wheeler N., Laverdiere M., Blazevic D., Wilkinson B. J. A new type of penicillin resistance of Staphylococcus aureus. Lancet. 1977 Feb 26;1(8009):443–447. doi: 10.1016/s0140-6736(77)91941-9. [DOI] [PubMed] [Google Scholar]
  18. Takebe I., Singer H. J., Wise E. M., Jr, Park J. T. Staphylococcus aureus H autolytic activity: general properties. J Bacteriol. 1970 Apr;102(1):14–19. doi: 10.1128/jb.102.1.14-19.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomasz A., Albino A., Zanati E. Multiple antibiotic resistance in a bacterium with suppressed autolytic system. Nature. 1970 Jul 11;227(5254):138–140. doi: 10.1038/227138a0. [DOI] [PubMed] [Google Scholar]
  20. Tomasz A. The role of autolysins in cell death. Ann N Y Acad Sci. 1974 May 10;235(0):439–447. doi: 10.1111/j.1749-6632.1974.tb43282.x. [DOI] [PubMed] [Google Scholar]
  21. den Kamp JA O. P., van Iterson W., van Deenen L. L. Studies of the phospholipids and morphology of protoplasts of Bacillus megaterium. Biochim Biophys Acta. 1967;135(5):862–884. doi: 10.1016/0005-2736(67)90056-9. [DOI] [PubMed] [Google Scholar]

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