Figure 1.

RT fusion constructs, encoded fusion proteins, and expression of fusions in yeast reporter strains. The six-alanine linker is denoted by the hatched box, and the HA epitope by black shaded regions. p66 and p51 indicate the 66- and 51-kDa subunits of HIV-1 RT, respectively. Expression of fusion proteins was determined by introducing the indicated plasmids into CTY10-5d, except for p66GBT9 and p51AS2-1, which were introduced into HF7c. Fusion protein expression was detected by probing yeast protein lysates with anti-RT antibodies, as described in Materials and Methods. ++, high; +, moderate; +/−, low; −, undetectable protein expression. ND, not done.