Figure 2.

Interaction of p66 deletion mutants with Gal4AD-HA-51 fusion protein. p66 domains were fused to the C terminus of lexA₈₇ in pSH2-1. CTY10-5d was cotransformed with the appropriate constructs. Transformants were lifted onto nitrocellulose and were subjected to β-gal colony lift assay to determine intensities of blue color produced as defined in Tables 1 and 2. β-gal activity from liquid assays is expressed in Miller units. Expression in CTY10-5d of p66 fusion proteins was detected by using anti-lexA polyclonal antibodies. Expression levels are as defined as in the legend for Fig. 1.