Table 2.

Interaction of p51 binding domain fusions with p66 activation domain fusion in the Y2H system

Constructs	Operator	β-gal activity
		Colony*	Liquid†
p51SH2-1 + pGADNOT	lexA	−	0.06
p51SH2-1 + pACTII	lexA	−	0.05
p51SH2-1 + p66AlaACTII	lexA	++	1.2
p51Lex202 + pACTII	lexA	−	0.05
p51Lex202 + p66AlaACTII	lexA	+++	3.2
p51AS2-1 + pACTII‡	UASG	−	ND
p51AS2-1 + p66AlaACTII‡	UASG	++	ND

Yeast strain CTY10-5d or ^‡HF7c were transformed with plasmids encoding p51 bait and p66 prey fusions. Fusion proteins encoded by plasmids are described in Materials and Methods and Fig. 1. 

^*As defined in Table 1. 

^† As defined in Table 1.