Table 3.

Second site mutations in lexA₂₀₂66HXL234A that restore dimerization to p51

Genotype	No. of clones	β-gal activity
		Colony*	Liquid†
Wild type	NA	+++	3.2
L234A	NA	−	0.1
D110G	NA	+++	6.9
W402R	NA	+++	7.1
W406R	NA	+++	5.8
L234A, D110G	3	+++	1.5
L234A; D186V	1	+/−	0.2
L234A; W402R	3	+++	7.1
L234A; W406R	1	+++	6.1
L234	3	+++	4.7

Yeast strain CTY10-5d was cotransformed with p51HXGADNOT and various clones expressing lexA₂₀₂-Ala-66HX fusions with mutations in p66 as indicated. NA, not applicable. 

*As defined in Table 1. 

^†As defined in Table 1.