Table 2 Analysis of DNA sequences of strains identified incorrectly using the Microseq 500 16S rDNA bacterial identification system database.
| Patient/strain no. | Identification by conventional 16S rRNA gene sequencing | Identification by DNA sequencing of first 527 bp fragment of 16S rRNA gene | ||||
|---|---|---|---|---|---|---|
| Using MicroSeq 500 16S rDNA database | Analysis using the GenBank database | |||||
| BM | No. of base (%) difference between strain and BM | 2nd BM | No. of base (%) difference between strain and 2nd BM | |||
| 4 | Clostridium disporicum | Clostridium paraputrificum | C disporicum | 10 (2.1) | Clostridium gasigenes | 24 (4.8) |
| 5 | Clostridium indolis | Clostridium innocuum | C indolis | 14 (2.7) | Clostridium symbiosum | 45 (8.7) |
| 6 | C innocuum | Eubacterium dolichum | C innocuum | 9 (1.7) | Eubacterium cylindroides | 17 (5.4) |
| 7 | Clostridium orbiscindens | Ruminococcus productus | C orbiscindens | 3 (0.6) | Bacteroides capillosus | 29 (5.8) |
| 11 | Clostridium septicum | Clostridium tertium | C septicum | 1 (0.2) | Clostridium chauvoei | 9 (1.8) |
| 15 | Eubacterium tenue | Clostridium tenue | Clostridium ghonii | 12 (2.4) | Clostridium bifermentans | 15 (3.1) |
| 19 | Olsenella uli | Atopobium rimae | O uli | 0 (0) | Olsenella profusa | 21 (4.3) |
BM, best match.