Figure 3 EpCAM immunohistochemistry performed on (A) normal oesophageal mucosa, (B–C) Barrett's metaplasia, and (D–F) normal gastric body mucosa. In particular, (B) demonstrates the sharp contrast in EpCAM expression shown by squamous and Barrett's epithelia. (D) Low power examination of gastric mucosa showed no obvious EpCAM immunostaining. (F) However, at higher power, scattered cells at the gland bases (the gland base shown in panel (E) is arrowed in panel (D)) were found to express EpCAM; these labelled cells had the morphological appearance of mucus secreting cells. (F) In contrast, neither chief (Ch) nor parietal (Pr) cells showed any immunostaining for EpCAM.