Table 2 Details of primary and corresponding secondary antibodies used for Western blotting.

Target protein (molecular weight)		Primary antibody type, clone and supplier		Primary antibody dilution, diluent,* and incubation conditions		Secondary antibody dilution
Actin (42 kDa)		Mono, AC‐15, Sigma		1:2000, 5% milk, 1 h at RT		1:10000
Cytokeratin 4 (59 kDa)		Mono, NCL‐LL002, Novocastra†		1:400, 5% milk, 1 h at RT		1:1000
EpCAM (40 kDa)		Mono, NCL‐ESA, Novocastra		1:25, 5% milk, 2 h at RT		1:1000
Villin (97 kDa)		Mono, NCL‐L‐Villin, Novocastra		1:1000, 5% milk, 1 h at RT		1:5000

Mono, monoclonal; RT, room temperature. *All diluents were based on phosphate‐buffered saline with 0.1% Tween 20; †Newcastle‐upon‐Tyne, Tyne and Wear, UK.