Figure 4.

CHIP analysis for acetylated histone association with LDL receptor and HMG CoA reductase promoters in sterol-depleted CHO-7 cells. Antibodies for acetylated H3 or H4 were used in a CHIP analysis followed by PCR for either the LDL receptor (A) or HMG CoA reductase (B) promoters, respectively. All symbols and notations are similar to Figs. 2 and 3. The starting chromatin was analyzed in lanes 1 and 2, and the primary antibody was left out of the incubations in lanes 3 and 4. Twenty cycles of PCR were used in both A and B. Serial dilutions of the input and precipitated material demonstrated that the PCR accurately reflected the amount of starting material as described in the legend to Fig. 2 (data not shown).