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| Target | Sequence (5′→3′) | Location | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| HP sense | aag ctt tta ggg gtg tta ggg gtt t | 784–809 (glu M) | 55 | 294 |
| HP antisense | aag ctt act ttc taa cac taa cgc | 1077–1053 (glu M) | ||
| MP sense | gcc acc ctg ggg ggc agt cag | 2599–2619 | 65 | 209 |
HP, Helicobacter pylori; MP, Mycoplasma pneumoniae.
