Figure 1 Evaluation of several factors in low‐grade and high‐grade prostate carcinoma (PCa). Immunostaining for Ki67 (A,E); CD34 (B,F); osteoprotegerin (OPG; C,G); and receptor activator of nuclear factor‐κ B ligand (RANKL; D,H) was performed with specific antibodies. Representative examples of a low‐grade PCa (A‐D) and a high‐grade PCa (E‐H) are shown. Negative controls (without corresponding primary antibody) exhibited no positivity (data not shown). Original amplifications, ×200.