Table 9 Immunohistological evaluation of the inflammatory cells in bullous skin lesions.
| DH | BP | BDE | CCD | EB | CBDC | LPA | ||
|---|---|---|---|---|---|---|---|---|
| CD68 | Total | 42.4 (5.80) | 31.0 (6.8) | 64 | 11 | 29.5 (5.50) | 43.5 (7.5) | 3.0 |
| Epidermis | 0.20 (0.20) | 0.40 (0.4) | 0 | 0 | 0.50 (0.50) | 0 (0) | 0 | |
| Bullae (inside) | 15.9 (3.00) | 5.40 (3.8) | 51 | 0 | 2.50 (0.50) | 7.50 (3.5) | 0 | |
| Bullae (around) | 0 (0) | 1.00 (0.6) | 0 | 0 | 0 | 9.00 | 0 | |
| DEJ | 3.20 (1.00) | 6.60 (2.9) | 0 | 5.0 | 6.00 (1.00) | 11.0 | 0 | |
| Perivascular | 28.2 (3.40) | 17.6 (3.5) | 13 | 6.0 | 20.5 (4.50) | 26.0 (10) | 3.0 | |
| CD20 | Total | 0.40 (0.40) | 0.40 (0.4) | 1.0 | 0 | 0.50 (0.50) | 0 (0) | 0 |
| Epidermis | 0 (0) | 0 (0) | 0 | 0 | 0 (0) | 0 (0) | 0 | |
| Bullae (inside) | 0 (0) | 0 (0) | 1.0 | 0 | 0 (0) | 0 (0) | 0 | |
| Bullae (around) | 0 (0) | 0 (0) | 0 | 0 | 0 | 0 | 0 | |
| DEJ | 0 (0) | 0 (0) | 0 | 0 | 0 (0) | 0 (0) | 0 | |
| Perivascular | 0.50 (0.50) | 0.40 (0.4) | 0 | 0 | 0.50 (0.50) | 0 (0) | 0 | |
| CD3 | Total | 37.3 (12.0) | 19.8 (6.7) | 45 | 27 | 22.5 (18.5) | 37.0 (5.0) | 50 |
| Epidermis | 0 (0) | 0 (0) | 0 | 0 | 0 (0) | 0 (0) | 0 | |
| Bullae (inside) | 2.70 (0.90) | 2.40 (1.3) | 21 | 7.0 | 10.5 (9.50) | 4.50 (2.5) | 0 | |
| Bullae (around) | 0 (0) | 0 (0) | 0 | 0 | 0 | 0 | 0 | |
| DEJ | 0 (0) | 0.60 (0.4) | 0 | 0 | 4.50 (4.50) | 3.50 (1.5) | 0 | |
| Perivascular | 34.7 (11.6) | 16.8 (6.4) | 24 | 20 | 7.50 (4.50) | 29.0 (1.0) | 50 | |
| TIA1 | Total | 16.4 (8.20) | 10.4 (2.6) | 10 | 21 | 7.50 (2.50) | 12.5 (2.5) | 30 |
| Epidermis | 0 (0) | 0 (0) | 0 | 0 | 0 (0) | 0 (0) | 0 | |
| Bullae (inside) | 2.00 (0.80) | 2.00 (0.9) | 8.0 | 4.0 | 1.00 | 1.50 (1.5) | 0 | |
| Bullae (around) | 0 (0) | 0.60 (0.4) | 0 | 1.0 | 0 | 0 | 0 | |
| DEJ | 1.20 (0.60) | 1.20 (0.4) | 0 | 1.0 | 1.50 (1.50) | 2.00 (0) | 0 | |
| Perivascular | 15.8 (9.00) | 6.60 (3.1) | 2.0 | 15 | 5.50 (1.50) | 9.00 (4.0 | 30 | |
| GRB | Total | 3.90 (2.10) | 3.20 (1.7) | 1.0 | 2.0 | 2.00 (2.00) | 1.50 (0.5) | 6.0 |
| Epidermis | 0 (0) | 0 (0) | 0 | 0 | 0 (0) | 0 (0) | 0 | |
| Bullae (inside) | 0.30 (0.20) | 0.20 (0.2) | 0 | 0 | 0 | 0 (0) | 0 | |
| Bullae (around) | 0 (0) | 0 (0) | 0 | 0 | 0 | 0 | 0 | |
| DEJ | 0.20 (0.20) | 0 (0) | 0 | 0 | 0 (0) | 0.50 (0.5) | 0 | |
| Perivascular | 4.00 (2.30) | 3.00 (1.6) | 1.0 | 2.0 | 4.00 | 1.00 (0) | 6.0 | |
BDE, bullous drug eruption (with subepidermal bullae); BP, bullous pemphigoid; CBDC, chronic bullous disease of childhood; CCD, chronic contact dermatitis; DEJ, dermoepidermal junction; DH, dermatitis herpetiformis; EB, epidermolysis bullosa; GRB, granzyme B; TIA1, T cell intracellular‐associated antigen 1.
Inflammatory cells were counted in three different fields (sections stained with haematoxylin and eosin, 400×). Values are expressed as mean (SEM).
Immunoreactivity score was evaluated as by other groups.12a,13 It was scored as mild, moderate or severe (scores 1, 2 or 3, respectively).