Figure 4.

Trt1p mutants are expressed at wild-type level. Cellular extracts prepared from S. pombe strains expressing untagged Trt1p and wild-type or mutant myc₉Trt1p were separated on an SDS polyacrylamide minigel, were transferred to a nylon membrane, and were probed with polyclonal anti-c-myc antibody. The putative band for the myc₉Trt1p fusion protein is indicated by an arrow. To confirm equal loading, identical samples were run on a second minigel, and proteins were stained with Coomassie blue (data not shown).