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Determination of transcription start site(s) of human Smad7 promoter. Poly(A)+ RNA (0.4 μg) from HaCaT cells that were untreated or treated with TGF-β (500 pM) for 1 h was used with a 22-bp oligonucleotide in the primer extension analysis. The products were analyzed on a denaturing gel (see A) with a sequencing reaction (not shown). The two transcription start sites (P1 and P2), the SBE, and the position of the 22-bp primer are marked.
