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. 2000 May 23;97(12):6403–6408. doi: 10.1073/pnas.120161397

Figure 2.

Figure 2

HPLC analysis of all-trans-RA metabolism in transfected COS-1 cells. Expression of either P450RAI-2 or P450RAI-1 in cells causes disappearance of all-trans-RA substrate (compare C with A or B) in addition to the generation of more polar metabolic products. Identities of the retinoids labeled as all-trans-RA, 4-OH-RA, 4-oxo-RA, and 18-OH-RA (A and B) were verified by coelution with known standards and comparison of the spectral properties by using photodiode array detection. Peaks labeled 1–4 (A) have spectral properties characteristic of retinoids, specifically UV maxima between 320 and 350 nm.