Figure 4.

Wild-type and H217Y Mre11 increase joining of nonhomologous and homologous ends. Plasmid DNA was cut with restriction enzymes to generate mismatched overhangs (XhoI/BamHI), cohesive 5′ overhangs (BamHI/BglII), or cohesive 3′ overhangs (SphI). The plasmids were incubated with varying amounts of wild-type or H217Y Mre11, as well as with ligase IV/Xrcc4 complex, as indicated. The products were amplified by PCR and run on a native polyacrylamide gel. Arrows indicate the positions of the uncut plasmid DNA and the joining products.