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In vitro polyadenylation of RNA I and tRNA variants by purified His-tagged PAP I. Reaction conditions were as in Fig. 1c. The corresponding RNA secondary structures are shown above each gel. (a) RNA I 42-mer and RNA I 31-mer. (b) GGG.RNA I, RNA I-t, supF tRNATyr, and tRNA-I. The percentage of adenylated substrate at each time point is plotted. RNA I (⧫), supF tRNATyr (■), RNA I-t (●), tRNA-I (○).
