Table 1 DNA oligonucleotides used in RT‐PCR sequencing.
Gene/exon | Sense/antisense | References | Sequence | Product size (bp) |
---|---|---|---|---|
Primer A | ||||
EWS/exon 7 | Sense | Ladanyi and Gerald3 | 5′‐tcctacagccaagctccaagtc‐3′ | |
WT1/exon 10 | Antisense | Ladanyi and Gerald 3 | 5′‐gccaccgacagctgaagggc‐3′ | |
Primer B | ||||
EWS/exon 8 | Sense | 5′‐catgagtggccctgataacc‐3′ | ||
WT1/exon 10 | Antisense | 5′‐accgggcaaactttttctg‐3′ | ||
Primer C | ||||
EWS/exon 7 | Sense | Ladanyi and Gerald 3 | 5′‐tcctacagccaagctccaagtc‐3′ | |
WT1/exon 8 | Antisense | Gerald et al2 | 5′‐accttcgttcacagtccttg‐3′ | |
IL‐2 | Sense | 5′‐aactcaccaggatgctcaca‐3′ | ||
Antisense | 5′‐gcacttcctccagaggtttg‐3′ | 105 | ||
IL‐2/15Rβ | Sense | 5′‐aatggcacttcccagttcac‐3′ | ||
Antisense | 5′‐agcagctcacaggtttggtt‐3′ | 146 | ||
JAK1 | Sense | 5′‐ggcgtcattctccaaagaag‐3′ | ||
Antisense | 5′‐aataggagccaggcatttca‐3′ | 140 | ||
STAT5 | Sense | 5′‐gtcctgaagacccagaccaa‐3′ | ||
Antisense | 5′‐gttgcgggtgttctcatttt‐3′ | 150 | ||
β‐actin | Sense | 5′‐aaactggaacggtgaaggtg‐3′ | ||
Antisense | 5′‐gtggcttttaggatggcaag‐3′ | 160 |
EWS, Ewing's sarcoma gene; JAK1, Janus kinase; STAT5, signal transducers and activators of transcription; WT1, Wilms's tumour gene.