FIGURE 1.
Acid/base titration of Δ+PHS (○) and Δ+PHS/V66D (•) monitored by changes in the intrinsic fluorescence of Trp-140. The line represents the fit of Eq. 1 or 2 to the data. All measurements at 298 K in 25 mM buffer, 100 mM KCl.
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