TABLE 2.

Thermodynamic stability of Δ+PHS/V66D determined by fluorescence-monitored GdnHCl titrations

pH	(kcal/mol)	Cm	m
2.96	0.63 ± 0.14	0.22	−2.92 ± 0.24
3.44	3.41 ± 0.04	0.80	−4.26 ± 0.04
3.90	5.52 ± 0.12	1.11	−4.97 ± 0.11
4.37	6.96 ± 0.08	1.29	−5.41 ± 0.06
4.84	7.26 ± 0.12	1.26	−5.78 ± 0.10
5.41	6.48 ± 0.14	1.16	−5.58 ± 0.12
5.96	5.69 ± 0.15	1.03	−5.55 ± 0.14
6.45	5.34 ± 0.12	0.95	−5.64 ± 0.12
6.99	4.93 ± 0.04	0.83	−5.95 ± 0.04
7.48	4.50 ± 0.04	0.77	−5.87 ± 0.04
7.95	3.71 ± 0.04	0.61	−6.06 ± 0.06
8.41	3.18 ± 0.08	0.54	−5.87 ± 0.11
8.90	2.77 ± 0.13	0.44	−6.25 ± 0.24
9.33	2.14 ± 0.09	0.37	−5.74 ± 0.19
9.77	1.18 ± 0.26	0.23	−5.20 ± 0.64
10.27	−0.36 ± 0.07	−0.06	−5.75 ± 0.10