TABLE 2.
ITC-derived thermodynamic parameters for the 27-mer quadruplex construct binding TMPyP4 in variable salt conditions at 25°C (298 K)
| [KCl] (mM) | ΔH1 (kcal/mol) | −TΔS1 (kcal/mol) | ΔH2 (kcal/mol) | −TΔS2 (kcal/mol) |
|---|---|---|---|---|
| 20 | −1.4 (±0.07) | −9.0 | −13.1 (±0.1) | 4.6 |
| 50 | −1.7 (±0.07) | −8.4 | −9.0 (±0.1) | 1.0 |
| 100 | −2.6 (±0.06) | −6.6 | −9.1 (±0.1) | 1.8 |
| 200 | −1.8 (±0.07) | −7.8 | −11.9 (±0.1) | 4.6 |
Parameters are for the two-independent-sites binding model. The uncertainties listed for ΔHi were determined by Monte Carlo analysis.