TABLE 3.
ITC-derived thermodynamic parameters for the 27-mer quadruplex construct binding TMPyP4 in 100 mM KCl at a range of temperatures from 5 to 45°C
| T (°C) | K1 (M−1) × 10−6 | ΔG1 (kcal/mol) | K2 (M−1) × 10−5 | ΔG2 (kcal/mol) |
|---|---|---|---|---|
| 5 | 26 (±9) | −9.4 | 2.2 (±0.1) | −6.8 |
| 15 | 12 (±3) | −9.3 | 2.7 (±0.1) | −7.2 |
| 25 | 5.4 (±0.5) | −9.2 | 2.0 (±0.1) | −7.3 |
| 37 | 1.7 (±0.4) | −8.8 | 1.1 (±0.1) | −7.2 |
| 45 | 3.7 (±0.8) | −9.5 | 1.3 (±0.1) | −7.4 |
Parameters are for the two-independent-sites binding model. The uncertainties listed for ΔHi were determined by Monte Carlo analysis.