TABLE 4.
ITC-derived thermodynamic parameters for the 27-mer quadruplex construct binding TMPyP4 in 100 mM KCl at a range of temperatures from 5 to 45°C
| T (°C) | ΔH1 (kcal/mol) | −TΔS1 (kcal/mol) | ΔH2 (kcal/mol) | −TΔS2 (kcal/mol) |
|---|---|---|---|---|
| 5 | −4.7 (±0.7) | −4.7 | −9.3 (±0.3) | 2.5 |
| 15 | −2.6 (±0.1) | −6.7 | −9.3 (±0.2) | 2.1 |
| 25 | −2.6 (±0.1) | −6.6 | −9.1 (±0.1) | 1.8 |
| 37 | −2.7 (±0.1) | −6.1 | −9.2 (±0.1) | 2.0 |
| 45 | −3.5 (±0.2) | −6.0 | −8.3 (±0.1) | 0.9 |
Parameters are for the two-independent-sites binding model. The uncertainties listed for ΔHi were determined by Monte Carlo analysis.